PUBLICATIONS

Reddy KD, Rasool B, Akher FB, Kutlešić N, Pant S, Boudker O. Evolutionary analysis reveals the origin of sodium coupling in glutamate transporters. Nat Struct Mol Biol. 2025 Aug 25. doi: 10.1038/s41594-025-01652-z. Epub ahead of print. PMID: 40855135.

Qiu B, Boudker O. Structural basis of excitatory amino acid transporter 3 substrate recognition. Proc Natl Acad Sci U S A. 2025 Apr 22;122(16):e2501627122. doi: 10.1073/pnas.2501627122. Epub 2025 Apr 18. PMID: 40249774; PMCID: PMC12036983.

Koochaki P, Qiu B, Coker JA, Earsley A, Wang NS, Romigh T, Goins CM, Stauffer SR, Boudker O, Chakraborty AA. Mechanism and Structure-Guided Optimization of SLC1A1/EAAT3-Selective Inhibitors in Kidney Cancer. bioRxiv [Preprint]. 2025 Jul 7:2025.07.03.663021. doi: 10.1101/2025.07.03.663021. PMID: 40672197; PMCID: PMC12265667.

Wang X, Rusinova R, Gregorio GG, Boudker O. Free fatty acids inhibit an ion-coupled membrane transporter by dissipating the ion gradient. J Biol Chem. 2024 Dec;300(12):107955. doi: 10.1016/j.jbc.2024.107955. Epub 2024 Nov 2. PMID: 39491650; PMCID: PMC11667161.

Jiang Y, Miyagi A, Wang X, Qiu B, Boudker O, Scheuring S. HS-AFM single-molecule structural biology uncovers basis of transporter wanderlust kinetics. Nat Struct Mol Biol. 2024 Aug;31(8):1286-1295. doi: 10.1038/s41594-024-01260-3. Epub 2024 Apr 17. PMID: 38632360; PMCID: PMC11490224.

Reddy KD, Rasool B, Akher FB, Kutlešić N, Pant S, Boudker O. Evolutionary analysis reveals the origin of sodium coupling in glutamate transporters. bioRxiv [Preprint]. 2024 Apr 25:2023.12.03.569786. doi: 10.1101/2023.12.03.569786. PMID: 38106174; PMCID: PMC10723334.

Drew D, Boudker O. Ion and lipid orchestration of secondary active transport. Nature. 2024 Feb;626(8001):963-974. doi: 10.1038/s41586-024-07062-3. Epub 2024 Feb 28. PMID: 38418916.

Fortea E, Lee S, Chadda R, Argyros Y, Sandal P, Mahoney-Kruszka R, Ciftci HD, Falzone ME, Huysmans G, Robertson JL, Boudker O, Accardi A. Structural basis of pH-dependent activation in a CLC transporter. Nat Struct Mol Biol. 2024 Apr;31(4):644-656. doi: 10.1038/s41594-023-01210-5. Epub 2024 Jan 26. Erratum in: Nat Struct Mol Biol. 2024 Apr;31(4):728. doi: 10.1038/s41594-024-01242-5. PMID: 38279055; PMCID: PMC11262703.

Qiu, B., Boudker, O. Symport and antiport mechanisms of human glutamate transporters. Nat Commun 14, 2579 (2023). https://doi.org/10.1038/s41467-023-38120-5

Huang Y, Reddy KD, Bracken C, Qiu B, Zhan W, Eliezer D, Boudker O. Environmentally Ultrasensitive Fluorine Probe to Resolve Protein Conformational Ensembles by 19F NMR and Cryo-EM. J Am Chem Soc. 2023 Apr 6. doi: 10.1021/jacs.3c01003.

Reddy KD, Ciftci D, Scopelliti AJ, Boudker O. The archaeal glutamate transporter homologue GltPh shows heterogeneous substrate binding. J Gen Physiol. 2022 May 2;154(5):e202213131. doi: 10.1085/jgp.202213131.

Ciftci D, Martens C, Ghani VG, Blanchard SC, Politis A, Huysmans GHM, Boudker O. Linking function to global and local dynamics in an elevator-type transporter. Proc Natl Acad Sci U S A. 2021 Dec 7;118(49):e2025520118. doi: 10.1073/pnas.2025520118.

Ciftci D, Huysmans GHM, Wang X, He C, Terry D, Zhou Z, Fitzgerald G, Blanchard SC, Boudker O. FRET-based Microscopy Assay to Measure Activity of Membrane Amino Acid Transporters with Single-transporter Resolution. Bio Protoc. 2021 Apr 5;11(7):e3970. doi: 10.21769/BioProtoc.3970.

Qiu, B., Matthies, D., Fortea, E., Yu, Z., & Boudker, O. (2021). Cryo-EM structures of excitatory amino acid transporter 3 visualize coupled substrate, sodium, and proton binding and transport. Science Advances, 7(10), eabf5814.

Huysmans GHM, Ciftci D, Wang X, et al. The high energy transition state of the glutamate transporter homologue GltPh. EMBO J (2020)e105415 . https://doi.org/10.15252/embj.2020105415

Wang X, Boudker O, Large domain movements through the lipid bilayer mediate substrate release and inhibition of glutamate transporters., eLife 2020;9:e58417, DOI: 10.7554/eLife.58417

Ciftci D, Huysmans GHM, Wang X, et al. Single-molecule transport kinetics of a glutamate transporter homolog shows static disorder. Sci Adv. 2020;6(22):eaaz1949. Published 2020 May 29. doi:10.1126/sciadv.aaz1949

Huang Y, Wang X, Lv G, et al. Use of paramagnetic 19F NMR to monitor domain movement in a glutamate transporter homolog. Nat Chem Biol. 2020;16(9):1006-1012. doi:10.1038/s41589-020-0561-6

Jentsch JA, Kiburu I, Pandey K, et al. Structural basis of sterol binding and transport by a yeast StARkin domain. J Biol Chem. 2018;293(15):5522-5531. doi:10.1074/jbc.RA118.001881

Riederer, E. A., Focke, P. J., Georgieva, E. R., Akyuz, N., Matulef, K., Borbat, P. P., Freed, J. H., Blanchard, S. C., Boudker, O., & Valiyaveetil, F. I. (2018). A facile approach for the in vitro assembly of multimeric membrane transport proteins. eLife, 7, e36478. https://doi.org/10.7554/eLife.36478

Scopelliti, A.J., Font, J., Vandenberg, R.J. et al. Structural characterisation reveals insights into substrate recognition by the glutamine transporter ASCT2/SLC1A5. Nat Commun 9, 38 (2018). https://doi.org/10.1038/s41467-017-02444-w

Oh, S., & Boudker, O. (2018). Kinetic mechanism of coupled binding in sodium-aspartate symporter GltPh. ELife, 7. doi: 10.7554/elife.37291

Ruan Y, Miyagi A, Wang X, Chami M, Boudker O, Scheuring S. Direct visualization of glutamate transporter elevator mechanism by high-speed AFM. Proc Natl Acad Sci U S A. 2017;114(7):1584-1588. doi:10.1073/pnas.1616413114

Drew, D., & Boudker, O. (2016). Shared Molecular Mechanisms of Membrane Transporters. Annual Review of Biochemistry, 85(1), 543–572. doi: 10.1146/annurev-biochem-060815-014520

Aseervatham, J., Tran, L., Machaca, K., & Boudker, O. (2015). The Role of Flexible Loops in Folding, Trafficking and Activity of Equilibrative Nucleoside Transporters. PloS one, 10(9), e0136779. https://doi.org/10.1371/journal.pone.0136779

Boudker, O., & Oh, S. (2015). Isothermal titration calorimetry of ion-coupled membrane transporters. Methods, 76, 171–182. doi: 10.1016/j.ymeth.2015.01.012

Akyuz, N., Georgieva, E. R., Zhou, Z., Stolzenberg, S., Cuendet, M. A., Khelashvili, G., … Blanchard, S. C. (2015). Transport domain unlocking sets the uptake rate of an aspartate transporter. Nature, 518(7537), 68–73. doi: 10.1038/nature14158

Verdon, G., Oh, S., Serio, R. N., & Boudker, O. (2014). Coupled ion binding and structural transitions along the transport cycle of glutamate transporters. ELife, 3. doi: 10.7554/elife.02283

Akyuz, N., Altman, R., Blanchard, S., & Boudker, O. (2013). Transport Dynamics in a Glutamate Transporter Homologue. Nature, 104(2). doi: 10.1016/j.bpj.2012.11.2983

Ginter, C., Kiburu, I., & Boudker, O. (2013). Chemical Catalysis by the Translocator Protein (18 kDa). Biochemistry, 52(21), 3609–3611. doi: 10.1021/bi400364z

Reyes, N., Oh, S., & Boudker, O. (2013). Binding thermodynamics of a glutamate transporter homolog. Nature Structural & Molecular Biology, 20(5), 634–640. doi: 10.1038/nsmb.2548

Georgieva, E. R., Borbat, P. P., Ginter, C., Freed, J. H., & Boudker, O. (2013). Conformational ensemble of the sodium-coupled aspartate transporter. Nature Structural & Molecular Biology, 20(2), 215–221. doi: 10.1038/nsmb.2494

Verdon, G., & Boudker, O. (2012). Crystal structure of an asymmetric trimer of a bacterial glutamate transporter homolog. Nature Structural & Molecular Biology, 19(3), 355–357. doi: 10.1038/nsmb.2233

Boudker, O., & Verdon, G. (2010). Structural perspectives on secondary active transporters. Trends in pharmacological sciences, 31(9), 418–426. https://doi.org/10.1016/j.tips.2010.06.004

Reyes, N., Ginter, C., & Boudker, O. (2009). Transport mechanism of a bacterial homologue of glutamate transporters. Nature, 462(7275), 880–885. doi: 10.1038/nature08616